Motivation and fields of application
Concentration determinations via measurements of light absorption or fluorescence are among the standard tasks in research, development and production monitoring in the bio, pharmaceutical and medical industries. However, the established analytical devices are often structurally oversized and cost-intensive for special tasks. In addition, many devices require samples to be taken and are therefore only suitable to a limited extent for continuous controls or examinations with a large sample throughput.
The sensor system developed contains four channels, each with an excitation LED, optical filters and detectors for measuring light transmission and fluorescence. The channels are designed to cover a wide range of the visible spectrum and can thus be used for the detection of many different substances. The system also contains an adjustable onboard signal amplification for low-noise data transmission. Depending on the fluorescent dye, concentrations down to the lower nMol/ml range as well as almost linear characteristic curves down to the mMol range could be demonstrated with the developed demonstrator.